血小板活化因子及其拮抗剂在大鼠严重腹腔感染中的作用

Role of platelet activating-factor and its antagonist in serious intra-abdominal infection of the rats

目的 探讨血小板活化因子（ＰＡＦ）及其拮抗剂在大鼠严重腹腔感染中的作用，及与肿瘤坏死因子（ＴＮＦ）和ＩＬ－８的关系。方法 应用大鼠盲肠结扎加穿孔模型，在不同时相点观察大鼠死亡率并检测其血ＰＡＦ、血清ＴＮＦ及ＩＬ－８的水平。结果①严重腹腔感染后，血ＰＡＦ、血清ＴＮＦ及ＩＬ－８水平均明显升高，且前者与后两者呈显著正相关。②应用ＰＡＦ拮抗剂ＷＥＢ２１７０治疗后，血ＰＡＦ、血清ＴＮＦ及ＩＬ－８水平可显著降低。③ＷＥＢ２１７０能明显降低严重腹腔感染大鼠的死亡率。结论 严重腹腔感染早期应用ＰＡＦ拮抗剂可阻挡ＰＡＦ与ＴＮＦ和ＩＬ－８的网络联系，对防治多器官功能衰竭具有重要意义。

Objective To investigate the role of platelet activating-factor (PAF) and its antagonist in serious intra-abdominal infection (SIAI) of rats, and the relationship between PAF and TNF or IL-8. Methods In a ligated and perforated cecum model of septic rat. The mortality and the level of blood PAF, serum TNF, IL-8 at different time points were observed. Results ①The level of blood PAF, serum TNF and IL-8 increased with a sig- nificant correlation between PAF and TNF/IL-8. ②After treated with PAF antagonist WEB2170, the level of blood PAF, serum TNF and IL-8 decreased with a reduced mortality rate. Conclusion The PAF antagonist WEB2170 can block the communication network of PAF with TNF or IL-8 and plays an important role in preventing MOF.