p16基因表达与胃癌临床生物学特征及预后的研究

Expression of p16 gene in Gastric Cancer and Its Correlation to Biologic Prognosis

目的：检测与分析p16基因表达产物在胃癌组织中的表达。探讨其与胃癌术后复发、转移及生存率的关系。方法：应用免疫组织化学方法检测84例胃癌标本中p16蛋白的表达。结果：84例中p16表达阳性率44．05％，转移淋巴结表达阳性率37．50％，明显低于正常组织及胃良性息肉。p16表达与组织学类型无关，与肿瘤浸润深度、临床分期及淋巴结转移有关。随访中发现，阴性表达者死亡率高、中位生存期短，转移复发率均高于阳性表达者(P＜0．01)。结论：p16蛋白异常表达在胃癌的发生发展过程中起重要作用。其可能成为临床评估肿瘤生物学行为及判断预后的标志之一。

Objective： To detect and analyse the expression of p16 gene in gastric cancer tissues and to explore its correlation with the postoperative recurrence， metastasis and survival rate of gastric cancer． Method： The expression of p16 in 84 gastric cancer patients were studied by immunohistochemistry and follow up survey． Results： The positive rate of p16 gene in 84 specimens of gastric cancer tissues was 44．05％ and its lymph node metastatic rate was 37，05％ which was lower than that in normal tissues (P＜0．01)． It was not correlated with its histologic type． However， but it was correlated with the lymph node metastasis， survival rate， and it invaded the various layers of stomach． Conclusion： Abnormal expression of p16 plays an important role in development of gastric cancer． p16 gene can be used to evaluate the tumor biologic behavior and the prognosis of disease．