不同肺癌细胞迁移和粘附能力的比较研究

Comparative Study on Cell Adhesion and Migration of Different Human Lung Carcinoma Cell

目的 研究和比较不同肺癌细胞迁移、粘附等生物学行为 ,探讨肿瘤细胞的转移机制。方法 将 4株肺癌细胞培养在含 1 0 %热灭活小牛血清、青霉素 (1 0 0U/ml)和链霉素 (1 0 0 μg/ml)、 37℃、 5 %CO2 下的RPMI1 640培液中 ,然后按DeanSheppard方法测定细胞的粘附能力 ;用划痕法和琼脂滴法测定细胞迁移能力 ;同时测定各肺癌细胞的铺展、软琼脂集落形成能力、梯形DNA和细胞生长曲线。结果 95C的迁移能力、细胞铺展率和增殖率最高 ,95D、A4次之 ,H4 6 0 最弱 ;95D的粘附能力最强 ,其次为 95C、A4 ;H4 6 0 的粘附率最低。只有H4 6 0 出现较强的梯形DNA ;各株细胞软琼脂集落形成能力无显著差异。结论 不同肺癌细胞迁移和粘附能力存在差异。提示肺癌细胞的转移能力可能与其生物学行为有关。

Objective To discuss the metastasis mechanic of tumor cell by means of comparing the biological behaviors like cell adhesion and migration of different human lung carcinoma cell metastases. Methods 4 human lung carcinoma cells were maintained in RPMI1640 supplemented with Penvicillin(100u/ml), treptomycin(100μg/ml) and 10% heat inactivated fetal bovin serum(FBS) in 37℃ and 5% CO 2, In vitro wound healing assays and agarose drop explant method were adopted to evaluate the migration and Dean Sheppard's method to evaluate cell adhesion.Cell spreading, colony forming growth curve and DNA ladder were studied too. Results 95 C listed the first in migration, cell spreading and proliferation with 95D the second, A4 the third and H 460 the last. 95D listed the first in adhesion ability 95C and A4 the second, H 460 the last. Only H 460 showed a strong DNA ladder. Forming ablities of every cell were almost the same. Conclusions Differences exist between cell adhesion and migration which may be related to their biologicl behavior.cell adhesion might be related to their biological behabior.