火棘提取物对血液凝固的影响

Effects of Pyracantha fortuneana Extract on Blood Coagulation

本实验对火棘浸膏和火棘浸膏的不同极性段进行了小鼠玻片法测凝血时间、体外血浆复钙时间和凝血酶原时间的研究,结果表明火棘浸膏灌胃给药可显著缩短小鼠的玻片凝血时间(P<0.05),体外给药可明显缩短血浆复钙时间(P<0.01)和血浆凝血酶原时间(P<0.01,P<0.05);而火棘浸膏的不同极性段中,氯仿相灌胃给药后缩短小鼠的玻片凝血时间(P<O.01)及体外给药缩短血浆复钙时间(P<0.01)和血浆凝血酶原时间(P<0.01)作用最为显著,其次是乙酸乙酯相(P<0.01,P<0.05),而石油醚相(P>0.05)无此作用,由此可筛选出火棘凝血活性部位在氯仿和乙酸乙酯相,石油醚相无凝血活性。

Objective: To study the effect of the Pyracantha fortuneana extract and its defferent polar fractions on blood coagulation. Methods and Results:The Pyracantha fortuneana extract could noticeably shorten the coagulation time of glass slide in mice (P < 0.05)after o-ral administradon, and also shorten the recalcification time and prothrombin time in vitro (P<0.01,P<0.05), while in three polar fractions of the extract, the effects of the chloroform phase that could shorten the coagulation time of glass slide in mice(P<0.01) after oral administration , and also shorten the recalcification time and prothrombin time in vitro (P<0.01) are more noticeable than that of the acetic ether phase (P<0.01, P<0.05). The petroleum ether phase has no above-mentioned effect (P> 0.05). Conclusion: The Pyracantha fortuneana extract can reduce the blood coagulation. Both the chloroform phase and the acetic ether phase of the extract have activity of blood coagulation, but the petroleum ether phase does not.