混合取代基-β-环糊精—毛细管电泳法分离测定麻黄碱和伪麻黄碱

Chiral Separation and Determination of Ephedrine and Pseudoephedrine with Dual Derivatization -β - Cyclodextrin System by Capillary Electrophoresis

目的:建立毛细管电泳法同时分离测定同分异构的2对手性化合物麻黄碱和伪麻黄碱的含量。方法:Waters CapillaryIon Analyzer,50μm×60cm空心熔融石英毛细管柱,柱上紫外检测。电泳条件:分离用缓冲液为25mmol·L^(-1)的磷酸-Tris 缓冲液,含18mmol·L^(-1)的羧甲基-β-环糊精和7.5mmol·L^(-1)的β-环糊精-硫酸酯,pH3.02。分离电压:18kV;温度:25℃;虹吸进样,高度10cm,时间1s;紫外检测波长214nm。结果:左旋伪麻黄碱、右旋麻黄碱、左旋麻黄碱和右旋伪麻黄碱线性范围分别为23.7～237μg·ml^(-1),25.0～250μg·mL^(-1),25.0～250μg·mL^(-1),25.4～254μg·mL^(-1),以信噪比等于3:1为标准,最小检测浓度均为5.0μg·mL^(-1);日内和日间精密度RSD在2.4％～4.3％之间。结论:方法分离效率高、简便、快速、成本低。

Objective: To set up a capillary electrophoresis method for the chiral separation and determination of pseu-doephedrine and ephedrine with dual derivatization -β- cyclodextrin system. Methods: The electrophoretic conditions were as follows: 50μm ×60 cm capillary column; buffer: 25 mmol·L-1 of phosphate solution containing 18 mmol·L-1 of CM -β- CD and 7.5 mmol·L-1 of SHE -β- CD, adjusted to pH 3.02 by trihydroxymethylaminomethane; detection wavelength: 214 nm; voltage: 18 kV; temperature: 25℃. Results: The established method was linear in the range of 25.0-250 μg·mL-1 of pseudoephedrine and ephedrine with good precision. The intra- day and inter- day precisions were between 2.4% and 4.3%. The detection limit ( S/N = 3:1) was 5.0 μg·mL-1. Conclusion: The method was efficient, accurate, fast and simple, and could be used for the chiral determination of pseudoephedrine and ephedrine.