柱前衍生HPLC法结合固相萃取测定血浆中卡托普利

Detection of Captopril in Human Plasma by Pre - column Derivation HPLC with Solid Phase Extraction

目的:建立测定血浆中卡托普利浓度的方法,用于卡托普利人体药动学及相对生物利用度研究。方法:以对溴苯甲酸甲基溴为衍生化试剂,固相萃取法从生物样本中提取纯化衍生化产物,以反相高效液相色谱法测定衍生物含量而确定卡托普利血药浓度。结果:卡托普利血药浓度在10～1000ng·mL^(-1)范围内线性良好,相关系数为0.9987,测得平均回收率为98.65％,日内、日间RSD均小于10％。结论:本方法简便、准确、干扰小,适合用于卡托普利血药浓度监测及药动学研究。

Objective: To establish a method to determine captopril concentration in human plasma by HPLC with solid phase extraction (SPE) for studying of the pharmacokinetic and the relative bioavailability of captopril tablets. Methods: Captopril in plasma was derivatized with p-bromophenacyl bromide, then the additional products sassing through SPE were determined by HPLC. Results: The linear range of plasma concentration was 5-1000 ng·mL-1 ( r -0.9987). The average recovery was 98.65% . The RSD for inter- day and intra- day were all less than 10% . Conclusion: The method is simple, accurate and sensitive, and will be suitable in determination of captopril in plasma and its pharmacodynamics.