+Gz重复暴露对兔脑组织c-fos蛋白表达的影响

Effects of Repeated +Gz Exposures on the Expression of c-fos Protein in Rabbit Brain.

目的观察 +Gz重复暴露后兔脑组织c fos蛋白表达与分布的变化。方法 2 0只新西兰兔随机分为4组 ,每组 5只。麻醉后行气管插管 ,左侧颈总动脉插管以记录眼水平动脉血压。 +Gz组动物暴露于+ 4G(增长率 1G/s)至眼水平动脉血压降为 0kPa后持续 30s ,重复暴露 3次 ,中间间隔 30min。对照组动物不进行 +Gz暴露。利用免疫组织化学方法观察 +Gz重复暴露后即刻、1h、6h及对照组动物术后 1h脑c fos蛋白表达的变化。结果对照组兔脑组织未见c fos阳性神经元。 +Gz重复暴露后即刻 ,兔脑皮质、海马、齿状回及第三脑室两侧即可见明显的c fos阳性神经元 ,暴露后 1h上述部位c fos阳性神经元数目达到高峰 ,暴露后 6h有所减少。结论 +Gz重复暴露 3次可诱导兔脑组织c fos表达 ,它可能在

Objective To investigate the effect of repeated +Gz exposures on the expression and distribution of c fos protein immunoreactivity in rabbit brain. Method Twenty rabbits were divided randomly into 4 groups (n=5 in each group). Animals were anesthetized and then exposed to +4 Gz until 30 s after the arterial pressure at eye level dropped to 0 kPa. The exposure was repeated for 3 times with 30 min intervals. The expression of c fos protein in rabbit brain was examined at 0 h, 1 h and 6 h after +Gz exposure by immunohistochemistry method. Result c fos protein was obviously expressed in cortex,the third ventricle, hippocampus and dentate gyrus immediately after repeated +Gz exposures, and strongly expressed 1 h after the exposure, and then had a tendency to decrease 6 h after the exposure. Conclusion Three +Gz exposures may cause time dependent c fos expression in rabbit brain, which may be involved in +Gz induced brain damage.