摘要
将猪的甲状腺球蛋白 (p TG) 10 0 μg/只分别于第 0 d、第 14d皮下注入 CBA小鼠体内 ,制作实验性自身免疫性甲状腺炎 (EAT)的动物模型。免疫干预组从 0～ 2 8d、治疗组从 10～ 38d单独或者联合应用环孢菌素 A (Cs A,10 m g/ kg)灌胃和 (或 ) 1,2 5 (OH) 2 D3 (0 .2 μg/ kg)腹腔注射。p TG免疫后第 2 8d、第 38d处死小鼠 ,取甲状腺组织作病理学检查 ,并检测血清中猪的甲状腺球蛋白抗体 (p TGAb)、猪的甲状腺微粒体抗体 (p TMAb)。免疫干预组和治疗组联合应用小剂量 Cs A和 1,2 5 (OH) 2 D3 分别使 EAT发病率降低 44 .44 %和 37.5 0 %,严重病例分别降低 71.43%和 6 0 .32 %。免疫干预组的血清 p TGAb、p TMAb的值均降低。提示 :小剂量免疫抑制剂 Cs A和 1,2 5 (OH) 2 D3 联合防治 EAT有效 ,并具有协同作用。
To investigate the combined use of low doses of cycloporin A (CsA) and 1,25-dihydroxyvitamin D 3 (1,25(OH) 2 D 3 ) in the prevention and treatment of experimental autoimmune thyroiditis (EAT), porcine thyroglobulin (100 μg) was injected into a CBA mouse on the day 0 and day 14 to establish the model of EAT. The immune prevention group from day 0 to day 28, and treatment group from day 10 to day 38 were daily administered CsA (10 mg/kg) intragastrically and/or 1,25(OH) 2 D 3 (0.2 μg/kg) intraperitoneally. After immunized by pTG, the mice were killed on day 28 and day 38, to examine the thyroid gland pathologically, and determine the levels of serum porcine thyroglobulin antibodies(pTGAb), porcine thyromicrosomal antibodies(pTMAb). The incidence of EAT in the immune prevention group and treatment group, with administration of low doses of CsA and 1,25(OH) 2 D 3 was decreased by 44.44 % and 37.50 % respectively, while that of severe cases in the two groups decreased by 71.43 % and 60.32 % respectively. The levels of serum pTGAb and pTMAb in the immune prevention group were lower than in the positive control group. These results suggested that two immunosuppressants of CsA and 1,25(OH) 2 D 3 , combined use at low doses might be an effective therapy for EAT.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2002年第1期65-67,共3页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
