聚合酶链反应对耐甲氧西林葡萄球菌的监测

PCR for a Surreillence Study on Methicillin-resistant Staphylococcus Areus

为了解同济医院临床各科室分离的金黄色葡萄球菌对甲氧西林类抗生素耐药性的状况 ,收集同济医院临床各科室 1999年分离的金黄色葡萄球菌 84株 ,通过设计的引物 ,利用扩增耐甲氧西林金黄色葡萄球菌的 m ec A基因 ,用地高辛标记的特异性探针与 PCR扩增产物杂交。 84株金黄色葡萄球菌中获得 8株扩增产物为阳性的菌株 ,用这些阳性产物与地高辛标记的探针进行 Southern blot分析 ,杂交结果与 PCR结果一致 ,故同济医院 1999年金黄色葡萄球菌对甲氧西林耐药率为 8.8%。将聚合酶链反应技术用于检测耐甲氧西林金黄色葡萄球菌 (MRSA) ,其敏感性、特异性较高 ,且重复性好 ,准确率比实验室常用纸片法更高 ,为指导临床应用抗生素提供了一个切实可行的方法。

The resistance of staphylococcus areus isolated from clinical departments of Tongji Hospital to methicillin was studied. MecA gene coding penicillin binding protein 2a (PBP 2a ) of staphylococcus areus was detected by PCR. Eight-four strains of staphylococcus areus were collected in 1999. MecA gene was amplified by PCR with designated primers. Southern blot was used to detect the PCR products.It was found there were 8 positive strains of MRSA by PCR in 84 strains of all the staphylococcus areus with the results of Southern blot being same as those of PCR test. In all the 84 strains of staphylococcus areus, the positive rate of multidrug resistance of staphylococcus areus (MRSA) was 8.8 %. When PCR is used to detect MRSA, its sensitivity and speciality is high. It can duplicate well, too. The PCR and Southern blot provide an available method to guide antibiotic appliance in clinical practice.