利用双水相乙醇-磷酸氢二钾体系萃取甘草有效成分的研究

Separation of Glycyrrhizic Acid by Aqueous Two-phase Extraction with EtOH-K_2HPO_4

研究了基于与水互溶的普通有机溶剂的双水相萃取体系 ,并考察不同种类盐分相能力的差异情况 ,根据分相后有机溶剂相的体积变化 ,选择乙醇 (EtOH) 磷酸氢二钾 (K2 HPO4)体系萃取甘草有效成分。确定萃取的最佳条件是 :当双水相体系的总量为 10mL ,V(EtOH)∶V(H2 O)=3∶2 ,磷酸盐的质量为 1 5 g ,在上述条件下 ,EtOH K2 HPO4体系的两相分配完全 ,分配系数(K)最大为 12 80 ,收率 (Y)高达 98 3%。

The phase separation ability of various salts and organic solvents for aqueous two phase extraction was studied.According to the variation of organic solvent phase volumes after separation,the partition behavior of glycyrrhizic acid in EtOH K 2HPO 4 systems was investigated.Under the optimum conditions V total =10 mL, V (EtOH)∶ V (H 2O)=3∶2 and m (K 2HPO 4)=1 5 g,the partition coefficient ( K ) can reach 12 80,and the yield of glycyrrhizic acid ammonium salt can reach 98 3%.