魔芋葡甘低聚糖醛酸双酯钠的合成

Synthesis of Konjac Oligo Hydroxypropanyl Glucomannuronate Sulfate Sodium

以固相多酶系统RT - 4酶解魔芋精粉制得的超滤低聚魔芋葡甘聚糖 (KGM) ,经酸性条件下氧化成低聚KGM醛酸。通过正交实验优化出双酯化条件 :羟丙基化 :m(低聚KGM醛酸 )∶m(环氧丙烷 ) =1 0 0∶1 2 5 ;硫酸酯化 :m(低聚KGM醛酸羟丙酯 )∶m(氯磺酸 ) =1 0∶2 5 ,分别恒温 30℃、6 8℃ ,搅拌反应 3h ,产品低聚KGM醛酸双酯钠对低聚KGM醛酸收率 76 4 % ,相对分子质量 2 0 0 0～ 4 0 0 0 ,结构经IR、1HNMR分析 ,按低分子肝素质量标准药检及药理实验 ,确证是一种新型类肝素药物。

Enzymolysis of refined konjac powder by immobilized enzyme RT-4 gave konjac glucomannun(KGM), which was oxidized to oligo glucomannuronic acid(Ⅰ)under acid condition and successively esterified with propylene oxide and chlorosulfonic acid.The esterification conditions were optimized by orthogonal experiments.In hydroxypropylation reaction, m (Ⅰ)∶ m (propylene oxide)=1 00∶1 25;in sulfation reaction, m (oligo hydroxypropyl glucomannuronate)∶ m (chlorosulfonic acid)=1 0∶2 5.The two reactions proceeded respectively at 30 ℃and 68 ℃ for 3 hours.Yield of the product with molecular weight 2000-4000 was 76 4%(base on Ⅰ). Structure of the product was characterized with IR and 1HNMR,and the pharmacological experiments were done in comparison with low molecular weight heparin.The results indicate that the product is a novel low molecular weight heparitin.