海绵状胶原膜真皮替代物的研制及实验研究

An experimental study on the dermal substitute of spongy collagen membrane

目的 探讨海绵状胶原膜作为真皮替代物的可行性。 方法 提取猪皮胶原 ,与 6 硫酸软骨素混合沉淀后冻干成膜。将胶原膜包埋于SD大鼠皮下 ,定期取材检测其组织相容性、血管化能力及降解速度。 结果 制备的胶原膜具有密集的相互贯通的微孔结构 ,且具有一定的强度与韧性 ,适于手术操作。SD大鼠皮下埋藏试验表明组织相容性较好 ,无明显急性炎症反应 ,血管化能力较强 ,降解速度慢 ,真皮构架稳定性好。 结论 海绵状胶原膜可作为真皮替代物移植于创面。

Objective To investigate the practicability of a spongy collagen membrane as a dermal substitute. Methods Porcine skin collagen was harvested and mixed and precipitated with chondroitin 6 sulfate,and then lyophilized for 48 hours to form sheets of highly porous membrane. The collagen membrane was embedded subcutaneously in SD rat′s skin. The histological compatibility, the vascularization degree and degradation status were determined periodically by means of tissue sampling. Results The collagen membrane possessed some degree of strength and tenacity with a structure consisting of multidinous interconnecting pores. It was easy to manipulate. Experimental subcutaneous embedding in SD rats indicated that the membrane exhibited good tissue compatibility, strong tendency to vascularization,with no evidence of acute inflammatory reaction,and slow degradation rate.Conclusion Spongy collagen membrane might be an optimal dermal substitute for wound coverage. [