烧伤后早期增生性瘢痕相关细胞骨架基因表达研究

A study of the expression of hypertrophic scar related cytosk eletal genes during early postburn stage

目的 筛选烧伤后早期增生性瘢痕细胞骨架相关基因 ,并探讨其在瘢痕挛缩产生中的作用。 方法 以包含 4 0 96种人类基因的基因芯片研究烧伤后早期增生性瘢痕组织中相关细胞骨架基因表达 ;并利用原位杂交技术观察其中一条基因在烧伤后早期增生性瘢痕组织中的定位分布表达。 结果 在 3例临床标本中 ,共找到瘢痕相关细胞骨架基因 13条 ,均表达上调。在烧伤后早期增生性瘢痕组织中 ,表达人成纤维细胞肌原蛋白基因的细胞明显增多。 结论 烧伤后早期增生性瘢痕组织中就发生了多种细胞骨架相关基因表达上调 ,这可能是烧伤后增生性瘢痕挛缩的重要原因。

Objective To screen the hypertrophic s car related cytoskeletal genes during early postburn stage, so as to explore the ir roles in postburn scar contraction. Methods cDNA microarray chips containing 4096 human cDNAs were employed to investigate the cytoskeletal gene expression of the scar samples fro m human postburn hypertrophic scar. Furthermore, the expression of one of the cy toskeletal genes in hypertrophic scar tissue was studied by in situ hybridizatio n. Results Thirteen up-regulated cytoskeletal genes in 3 early post burn hypertrophic scar samples were identified. Moreover, the cells expressing h uman tropomyosin TM30 mRNA, one of the upregulated cytoskeletal genes, were fo und increased in the early postburn hypertrophic scar samples . Conclusion In this study upregulated expression of many hypert rophic scar related cytoskeletal genes was found in the scar samples during earl y postburn stage, and they might be important factors leading to postburn hypert rophic scar formation and contraction.