大段同种异体骨骨折修复的过程与特征

Experimental study for fracture repair of massive allografts

目的观察大段同种异体骨骨折修复过程和特征。方法采用新西兰大白兔32只切除股骨干和骨膜2cm,植入深低温保存的中间被截断的同种异体骨,用直径3mm髓内针固定,术后2、4、8、16周分别行X线摄片、组织学、四环素荧光标记检查。结果异体骨骨折修复是建立在异体骨活化基础上,术后2、4周骨折端不形成骨连接,8、16周两骨折端及周围均被纤维组织、软骨细胞、编织骨填充,从而构成内、外骨痂连接,但显著低于异体骨与宿主骨的结合部(P<0.05)。结论异体骨骨折修复与异体骨的活化密切相关,术后异体骨周围电磁场刺激等,促进了异体骨活化,有利于骨折修复。

Objective To study the mechanism and characteristic for fracture healing of massiv e allografts.Method The animal models of bone massive defect were ma de in 32New Zealant white rabbits.Wh ose femur shafts were removed 2cm of d iaphisis and pe-riosteum.The bone defect were fille d by the similar segment from frozen a llografts.A transverse osteotomy w as made in the middle of the allografts.Animal models for fracture of massive allografts have been e stablished and then the fracture segment were fixed with 3mm in-tramedullary triangle nailing.Obeservations were with X-ray,histolo gical and tetracycline-labled exam ination at 2,4,8,16weeks post-operative.Results The processes of fracture repair of m assive allografts were on the basis o f activable of bone allogeneic,the u nion can' t form at the fracture junction at 2,4weeks postoperative,the fracture j unction and its periphery were fille d by the fibrous tissue,chon-droblast,woven bone,they formed th e intramedullary and outmedullary c allus at 8,16weeks postoperative.T he distal and the porximal host-donor junction have callus sin gnificantly more than the fracture j unction(P<0.05).Conclusion The fracture repair of allograft have relation to generally activabl e of bone allogeneic.New bone formation was late and slow,moreover mostly in the form of endochondral ossification.