3种药物体外抗细粒棘球蚴原头节作用的比较

COMPARATIVE STUDY ON THREE DRUGS AGAINST ECHINOCOCCUS GRANULOSUS PROTOSCOLECES IN VITRO

目的 通过比较氧苯达唑、阿苯达唑和阿苯达唑脂质体体外抗细粒棘球蚴原头节作用 ,探讨氧苯达唑抗包虫病的作用。 方法 将氧苯达唑、阿苯达唑和阿苯达唑脂质体分别配成高、中、低浓度加入 RPMI16 4 0培养基中 ,体外培养细粒棘球蚴原头节 ,观察其每天的死亡率 ,直到对照组的头节全部死亡为止。 结果 将相同条件下 3种药物作用原头节的死亡率分别与对照组相比 ,阿苯达唑、氧苯达唑高、中、低浓度组与对照组相比 ,差异均有统计学意义 (P<0 .0 5 ) ;而阿苯达唑脂质体仅在高浓度时与对照组的差别有统计学意义 (P<0 .0 5 )。 结论 氧苯达唑、阿苯达唑和阿苯达唑脂质体均有显著的体外抗细粒棘球蚴原头节作用 ;氧苯达唑体外抗细粒棘球蚴原头节作用与阿苯达唑相当 ,可认为是一种新型抗包虫药 ;阿苯达唑脂质体剂型并未显示出特殊的体外抗原头节作用。

Objective Anti hydatid efficacy for Echinococcus granulosus protoscoleces( Eg PSC) was investigated by using Oxibendazole (OBZ), Albendazole (ABZ) and Liposomal Albendazole (L ABZ) in vitro . Methods Eg PSC was cultured in three drugs concentrations 2 500 ng/ml, 500 ng/ml, 100 ng/ml) of OBZ, ABZ and L ABZ in the RPMI1640 culture media respectively. The mortality in each group was daily counted until 100% Eg PSC death in control. Results Significant difference of Eg PSC mortality was found among these three drugs with various concentrations compared to control group ( P <0.05); In L ABZ group, however the statistical difference was found only in high concentrations( P <0.05). Conclusion The significant anti protoscolece effect for these three drugs was found in our current experiment in vitro . OBZ had the similar anti hydatid effect compared with ABZ in vitro , which could be considered as a potential candidate drug against E. granulosus . L ABZ did not demonstrate the significant effect of anti hydatid Eg PSC in vitro .