天然秸秆纤维素分解菌的分离选育

Isolation and Screening on Straw Cellulose-Decomposing Microorganisms

从土壤、酒糟、腐烂的玉米秸秆及牛粪中共分离到14株能分解纤维素的真菌菌株,外加已保藏的10株共24株菌株。分别对其进行了滤纸分解度、羧甲基纤维素(CMC)酶活和天然纤维素酶活的测定。筛选出6株对天然纤维素有较强的降解能力,酶活力在60mg/mL.d以上的菌株。通过改变其培养基中天然纤维素的含量,发现随着培养基中天然纤维素含量的减少,酶活力也随之降低。

24 strains of fungi for decomposing cellulose were chosen, including 14 isolated from soil, distiller's grains, putrid corn straw and cattle feces. On the basis of these strains, 6 suitable strains were obtained through the experiments of decomposition of filter paper, determinations of CMC enzyme activity and natural cellulase activity, The experimental results showed that their enzyme activity of decomposing natural cellulose reached over 60mg/ml?