胎肝细胞宫内胎鼠腹腔注射对其移植免疫反应的影响

Effect of intraperitoneal injection of fetal liver cells into the fetus of pregnant mice on the transplantation immunologic reaction of mice

目的 研究胎肝细胞宫内注射对小鼠移植免疫反应状态的影响 .方法 利用 C5 7BL /6小鼠的胎肝细胞对BAL B/c胎鼠行宫内胎鼠腹腔注射 (孕期第 14～ 16日 ) ,分娩存活的 BAL B/c雌性小鼠 7～ 9wk时作受体 ,C5 7BL/6新生鼠作供体 ,行异位心肌移植 ,以小鼠心肌移植后的存活时间、混合淋巴细胞培养和对诱导迟发型变态反应 (DTH)的影响观察该方法对小鼠移植排斥反应的影响作用 .结果 和对照组相比较 ,宫内注射组小鼠移植后心肌存活时间明显延长 ,混合淋巴细胞培养等结果也具有显著性差异 (P<0 .0 1) .结论 胎肝细胞宫内注射的方法可以延长小鼠心肌移植的存活期 。

AIM To investigate the effect of transplantation immunologic reaction of mouse by fetal liver cells utero injection. METHODS C57BL/6 fetal liver cells were injected intraperitoneally into the fetus of pregnant BALB/c mice at day 14 to 16 of gestation. At 7～9 weeks age, the surviving fe male fetus received transplantation of C57BL/6 newborn cardiac tissue grafts. Mice's cardiac tissue graft survival time after transplantation, mixture lymphocytes culture and delayed type hypersensitivity were observed to confirm the anti rejection effect of the method of fetal liver cells utero injection. RESULTS As compared to those in the control groups, the cardiac tissue graft's survival time after transplantation of the fetal liver utero injection group were prolonged ,and the other rejection datas of the utero injection group were significantly different from those of controls ( P <0.01). CONCLUSION Method of fetal liver cells utero injection can prolong the survival time of mice cardiac tissue graft. The suppression effect on rejection is efficient.