应用显微切割TRAP法对食管癌端粒酶活性进行分析

Analysis of telomerase activity in esophageal carcinoma using microdissection telomeric repeat amplification protocol assay

目的 探讨端粒酶活性在食管粘膜上皮不典型增生、食管鳞癌中的变化 ,同时研究端粒酶活性与癌的分化、浸润、转移的关系。方法 应用显微切割 TRAP 银染法对 4 5例食管鳞癌组织、癌旁非典型增生上皮及切缘的正常粘膜上皮进行定点端粒酶活性的检测。结果 食管正常粘膜上皮的端粒酶活性阳性率为 5 % (2 / 4 0 ) ,非典型增生上皮为 79 3% (2 3/ 2 9) ,癌组织为 82 2 % (37/ 4 5 )。非典型增生上皮的端粒酶活性明显高于正常上皮 ,差异有显著意义 (P <0 0 1)。同一癌组织中不同分化程度、不同浸润深度癌巢的端粒酶活性差异无显著意义 (P >0 0 5 )。有淋巴结转移者癌组织端粒酶活性明显高于无淋巴结转移者 ,差异有显著意义 (P <0 0 5 )。结论 食管鳞癌及癌旁不典型增生组织端粒酶活性明显增高。癌组织中端粒酶活性与分化程度无关 。

Objective To investigate the changes of telomerase activities in atypical hyperplasia of esophageal mucosal epithelium and esophageal squamous cell carcinoma (SCC) and to study the association of telomerase activity with differentiation, invasiveness, and lymphatic metastasis of cancer. Methods The telomerase activities of esophageal SCC tissues, adjacent tissue with dysplasia, and normal mucosal epithelium from surgical edge of 45 cases were detected by microdissection-TRAP(Telomeric Repeat Amplification Protocol) silver assay. Results The telomerase activity rates were 79.3% (23/29) in atypical displastic epithelium, 82.2% (37/45) in SCC tissue, and only 5% (2/40) in normal epithelium. The difference of telpmerase activity between dysplastic and normal esophageal epithelia was highly significant ( P <0.01). In the same cancer tissue the differences of telomerase activity among cancer nests to different degrees of defferentiation and to different depths were not significant ( P >0.05). The positive rate of telomerase activity was significantly higher in cases with lymphatic metastasis than in cases without lymphatic metastasis ( P <0.05). Conclusion The telomerase activities is increased in esophageal SCC tissue and adjacent atypical dysplastic tissue. The telomerase activity in SCC tissue is related to lymphatic metastasis but not related to cancer differentiation.