抑癌基因p16在肺鳞癌和肺腺癌中的表达及临床意义

Expression of P16 Gene in Primary Squamous Cell Carcinomas and Adenocarcinomas and its clinical significanhe

目的 探讨 p16基因产物在肺鳞癌和肺腺癌中的表达及其意义。方法 本组 5 6例原发性非小细胞肺癌 ,其中鳞癌 37例 ,腺癌 19例。用免疫组织化学 PCR法检测患者肺癌新鲜标本 p16蛋白表达水平。结果 5 6例肺癌标本中 p16蛋白阳性表达率为5 8.9% (33/ 5 6 ) ,伴有淋巴结转移者其阳性表达率 41.4% (12 / 2 9)显著低于无淋巴结转移者 (P16阳性表达率为 77.8% (2 1/ 2 7) ,P<0 .0 1)。P16蛋白阴性表达者的 1年、3年生存率分别为 5 9.7%、44 .1% ,显著低于 p16蛋白阳性表达者 85 .2 %、71.8%。结论 p16蛋白表达与肺鳞癌和肺腺癌的组织类型 ,淋巴结转移及预后有关。 p16蛋白状态可作为判断肺癌预后的指标之一。

objective To study the expression of p16 gene in primary squamous cell carcinomas and adenocarcinomas,and to determine the relationship between the expression of the gene product and lung cancer.Methods Usingb primers to amplify exon 2 of p16 gene by PCR to analyze the homozygous deletion of the p16 gene in 56 patients with NSCLC from 1996.2 to 2000.6m,including 37 squamous carcinomas,and 19 adenocarcinomas.We measured the correlation between clinicopathologic factors and homozygous deletion of the p16 gene in 56 cases.Results 33 cases showed positive stainign in all(58.9%).The expression level of p16 were higher in adenocarcinomas and squamous cell carcinomas without hilar or mediastinal lymph node metastasis 21/27(77.8%) than those with hilar or mediastinal lymph node involvement 12/29(41.4%)(P<0.01).Follow-uo showed that 1a,3a survival in p16 positive patients was 59.7% and 44.1% respectively ,which was much lower than in p16 negative patients(85.2%,and 71.8%)respectively.Conclusion Differentiation degree lymph node metastasis and prognosis of lung cancer were associated significantly with p16 expression.P16 expression was somewhat related to clinical stage.P16 expression may be used to effectively monitor the course of disease and to suggest prognosis of lung cancer.