摘要
探讨胃癌组织显微自体荧光图像的特征表现。方法：采用氖离子（Ａｒ＋）激光（激发波长 ４８８ｎｍ）和氦氖(Ｈｅ－Ｎｅ）激光（激发波长５４３ ｎｍ）的双通道法激光扫描共聚焦显微镜对１６例胃癌手术标本进行自体荧光图像分析，井作自身对照研究。结果：胃正常组织各层的显微自体荧光图像均以绿色荧光为主。胃癌的显微自体荧光图像则红色荧光强度明显增加，并可见棕红色区，其显示率达１００％。结论：棕红色区是胃癌在显微自体荧光图像上的特征表现，自体荧光图像分析是胃癌诊断的有效方法。
Background/Aims: To investigate the characteristics of autofluorescence image in gastric can- cer. Methods: A double-channel laser scanning confocal microscopy with Argon ion laser(excitation wavelength 488 nm) and Helium-Neon laser(excitation wavelength 543 nm) were used to detect the autofluorescence from 16 surgical specimens of gastric cancer and corresponding normal tissue. Re- sults: Autofluorescence emitted from normal gastric tissue appeared as a green image. The intensity of red color increased obviously in all gastric cancer tissue(100%) after illuminatin and it appeared as a brown reddish image. Conclusions: Brown reddish image is the characteristics of autofluorescence in gastric cancer excited by Argon ion laser and Helium-Neon laser with a double-channel laser scanning confocal microscope. Autofluorescence imaging analysis is useful in the diagnosis of gastric cancer.
出处
《胃肠病学》
2002年第1期11-13,共3页
Chinese Journal of Gastroenterology
