荧光定量检测血清HBV-DNA与HBeAg、临床类型的关系

目的 了解乙型肝炎病毒携带者、慢性乙型肝炎患者病毒含量与血清HBeAg、临床类型的关系。方法 采用荧光定量聚合酶链反应检测乙型肝炎病毒携带者和慢性乙型肝炎病人血清HBV -DNA含量。 结果 乙型肝炎病毒携带者HBeAg阳性组HBV -DNA含量明显高于HBeAg阴性组 ;乙型肝炎病毒携带者HBeAg阴性组中有25%HBV -DNA呈阳性 ,少数病例HBV -DNA水平还很高 ;乙型肝炎病毒携带者HBV -DNA含量明显高于慢性乙型肝炎。 结论 血清HBeAg是反映乙型肝炎病毒复制的良好指标 ,阳性乙型肝炎携带者HBV -DNA含量低于乙型肝炎病毒携带者。

Objective To explore the relationship between serum HBV-DNA and serum HbeAg and clinical types in HBV health carriers and patients with chronic hepatit B. Methods Serum HBV-DNA was detected with fluorescence quantitative PCR in HBV health carriers and patients with chronic Hepatitis is B. Results The HBV-DNA level in HBeAg-positive HBV health carriers was obviously higher than that in HBeAg-negative carriers. HBV-DNA was positive in 25% of HBeAg-negative carriers, and its level was even quite high in a few of such carriers. The HBV-DNA level in HBV health carriers was significantly higher than that in patients with chronic Hepatitis B . Conclusions Serum HBeAg is an excellent marker to reflect the replication of HBV . The HBV-DNA level in HBeAg-positive HBV health carriers was obviously lower than that in HBeAg-negative carriers.