氧电极法测定血清葡萄糖方法学探讨

An approach into Oxygen-electrode Method in determination of Serum Glu-cose.

目的了解电极法测定血清GLU的实验特点。方法采用氧电极法测定血清葡萄糖,并对其方法学进行了初步研究。结果氧电极法测定血清葡萄糖批内精度≤1.01%,批间精度≤1.74%,线性范围达46.81mmol/L,平均回收率101.08%,反应特异,快速,干扰因素少,本法(Y)与葡萄糖氧化酶法(X1)和己糖激酶法(X2)比较:Y=0.9922X1+0.1045,γ1=0.9997,P1>0.05;Y=0.9966X2-0.0278,γ2=0.9994,P2>0.005。结论氧电极法测定血清葡萄糖准确,快速,适合临床应用。

Objective To understand the characteristics of oxygen -electrode method in determination of serum glucose.Methods serum glucose was determined by oxygen-electrode method.Results In this method,within-run and between-run CVs were≤1.01%and≤1.74%respectively.The linear range of glucose concentration was up to46.81%mmol/L.The mean rate of recovery was101.08%.This method was more spe-cific and rapid.comparing with GOD method(X 1 )and HK method(X 2 ),yield a regression equation of Y=0.9922X 1 +0.1045,γ 1 =0.9997,P 1 >0.05;Y=0.9966X 2 -0.0278,γ 2 =0.9994,P 2 >0.05.Conclusion It has advantages of specific reaction and rapid report for clinical ap-plication.