活络舒肝胶囊对急性肝损伤细胞保护作用的实验研究

The Experimental Study of Protective Effect of Huoluoshugan Capsule on Acute Hepatic Injury

目的 :研究活络舒肝胶囊对急性肝损伤的肝细胞保护作用。方法 :SD大鼠 30只均分为 3组 :模型组采用四氯化碳腹腔注射制作急性肝损伤模型 ;治疗组首先予以活络舒肝水溶液 6 m l/ d,分 3次灌胃 ,1周后造模 ,造模后继续给予此药灌胃 ;另设正常组比较。造模 1周后取肝组织行组织病理学检查 ,同时采集静脉血进行血浆肝酶学和血蛋白质含量的测定。结果 :模型组大鼠出现明显的肝细胞坏死 ,而治疗组大鼠坏死范围明显缩小 ,程度明显减轻 ,此外 ,电镜检查结果两组亦有差异。模型组大鼠丙氨酸氨基转移酶、门冬氨酸氨基转移酶、碱性磷酸酶值升高 ,总蛋白下降 ,与治疗组相比差异有显著性意义 (P <0 .0 5 )。结论

Objective:To investigate the protective effects of Huoluoshugan capsule on hepatocytes in the rats with acute hepatic injury and its mechanism. Methods: Acute hepatic injury model was made by injecting CCl 4 into celiac cavithy.Thirty SD rats were randomly divided into three groups: normal group, model group and treatment group.In the treatment group, solution of Huoluoshugan was given orally to the rats, 6 ml per rat every day and a week later model of acute hepatic injury was induced as above. One week after making the model pathologic change of the liver tissue was observed under a light microscope and an electron microscope. The levels of serum ALT,AST,ALKP,TP and ALB were measured.Results: Huoluoshugan capsule could improve the pathologic change of liver tissue of acute hepatic injury and significantly decrease the levels of ALT,AST,ALKP. Conclusions: Huoluoshugan capsule has protective functions on rats with acute liver injury.