胶原海绵与兔软骨细胞体外三维培养的实验研究

The experimental study on three-dimensions culture of rabbit chondrocytes seeded into type I collagen sponge of human placenta

目的 观察用人胎盘Ⅰ型胶原海绵作为兔软骨细胞体外三维培养支架时 ,软骨细胞形态学特征和功能及该支架的吸附性和组织相容性。方法 将新生兔原代和传代软骨细胞接种于人胎盘Ⅰ型胶原海绵进行体外培养 ,用光镜和扫描电镜观察细胞形态和结构及支架的吸附性和组织相容性 ,用免疫组化观察细胞Ⅱ型胶原合成。结果 以胶原海绵作为支架的传代软骨细胞能维持其形态学特征及细胞功能 ,该支架有较好的吸附性和组织相容性。

Objective To observe the morphology and function of chondrocytes seeded into type I collagen sponge of human placenta and to find the sponge's adsorption to chondrocytes and it's biocompatibility Methods Primary and passage chondrocytes of newly born rabbits were cultured after they were seeded into type I collagen sponge of human placenta.The morphology and structure of chondrocytes cells,as well as sponge's adsorption and it's biocompatibility were observed by using light microscope and scanning electron microscope.The synthesis of collagen of type Ⅱ was studied by using immunohistochemical method.Results Passage chondrocytes seeded into the sponge maintained the morphology characteristic and cells functions.The sponge had excellent adsorption and biocompatibility.Conclusion The type I collagen sponge of human placenta can be used as the scaffolds in the three dimensions culture of chondrocytes.