银杏叶提取物对实验大鼠脑外伤后神经细胞凋亡的影响

The effect of Ginkgo biloba extract on rat neurons apoptosis after traumatic brain injury

目的 观察银杏叶提取物 (EGb761)对实验大鼠脑外伤后神经细胞凋亡的影响 ,探讨EGb761对脑外伤的治疗作用。方法 采用Feeney法自由落体撞击脑损伤动物模型 ,将 90只大鼠随机分为研究组及对照组 ,在损伤后15min ,60min及 2 4h分别腹腔注射EGb761及生理盐水。第 5天用TUNEL法测定神经细胞原位凋亡、大脑半球含水量、光镜下观察细胞形态学改变。结果 与对照组比较 ,大鼠脑外伤后 15min及 60min给予EGb761能显著降低神经细胞凋亡率和脑组织含水量 (P <0 0 5 ) ,外伤后 2 4h给药 ,神经细胞凋亡率和脑组织含水量差异无显著性 (P >0 0 5 )。结论 EGb761有抗大鼠脑外伤后神经细胞凋亡及减轻脑水肿的作用 。

Objective To investigate the effect of Ginkgo biloba extract (EGb761) on rat neural cell apoptosis after brain trauma. Methods Brain trauma was induced by Feeney's free-falling method in situ cerebral deathkit. Ninety SD rats, divided into control and trial group, were treated with EGb761 or saline i.p. 15 min, 60 min and 24 h after brain injury. Five days later, apoptosis was evalued by TUNEL method. Cerebral water content and cell morphology were elevated. Results Compared with the control-group, EGb761 given 15 min and 60 min after brain trauma attenuated neural cell apoptosis and brain edema. When given 24 h after brain trauma, however, EGb761 showed no significant effect. Conclusion Early large dosage of EGb761 can relieve traumatic brain edema and protect neural cells from apoptosis.