兔内毒素血症时sCD_(14)、E-选择素和IL-10水平及其与预后的关系

Dynamics of Serum Levels of Soluble CD_(14) ,E-selectin and Interleukin-10 in Rabbits with Endotoxemia

目的 探讨血清可溶性CD14 (sCD14 )、E 选择素、白细胞介素 10 (IL 10 )、肿瘤坏死因子 α(TNF α)和平均动脉压 (MAP)在内毒素血症病理变化过程中的水平及其相互关系。方法 复制兔内毒素血症模型 ,分别于模型制作后 0min、30min、6 0min、12 0min、180min、2 40min及 30 0min取血测定E 选择素、IL 10、sCD14 及TNF α含量 ,同时八导生理记录仪记录MAP。结果 内毒素血症组中TNF α及sCD14 含量 30min后即开始增加 ;E 选择素和IL 10含量 12 0min后明显增加 ;MAP则于 12 0min后明显下降。对照组中各指标水平变化不明显。结论 sCD14 、TNF α、E 选择素及IL 10在内毒素血症的发病机理中起着重要的作用。sCD14 和TNF α是反映感染病理变化早期过程中的重要指标之一 ;E 选择素和IL

Objective To study the change in serum levels of soluble CD 14 , tumor necrosis factor α, E selectin, interleukin 10 and mean arterial pressure, as well as their relationship to infection during the pathophysiologic process in endotoxemia of rabbits. Methods Sixteen rabbits were randomly divided into two groups: group A, as a control group; group B, endotoxemia group. The model of rabbit with endotoxemia were used. Endotoxin at a dose of 1.5 mg/ (kg·h) or 3 mg/(kg·h) was continuously infused through external jugular vein within 2 hours, 1 hour respectively. The change of levels of serum soluble CD 14 , tumor necrosis factor α, interleukin 10 and E selectin were observed at 0 (time before infusion of endotoxin), 30, 60, 120, 180, 240, 300 minutes, while mean arterial pressure was measured by polygraphy system. Results In the group B,there was an increase of content of soluble CD 14 ,tumor necrosis factor α,interleukin 10 and E selectin following 30, 120 minutes respectively,and mean arterial pressure was lower than that of group A at same time points. Conclusion The results suggest that soluble CD 14 ,tumor necrosis factor α,interleukin 10 and E selectin may play an important role during the change of infection and that these changes may be closely related with severe infection.