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持续性CO_2气腹影响腹腔镜手术切口肿瘤细胞种植的实验研究 被引量:5

An Experimental Research on the Effect of Continuous CO_2 Pneumoperitoneum on Tumor Cell Port Site Implantation in Laparoscopic Surgery in a Murine Model
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摘要 目的 探讨腹腔镜肿瘤外科手术中CO2 气腹对肿瘤细胞切口种植的影响。方法 雄性SD大鼠腹腔内注入不同浓度的人胃癌细胞株SGC 790 1,气腹机分别产生不同腹腔压力 [15mmHg/30mmHg( 1mmHg =0 .133kPa) ]的持续性气腹 ,通过 5mm腹腔穿刺管收集气体至培养瓶中 ,在 37℃、5 %CO2 环境中培养 7天后 ,涂片鉴定是否有肿瘤细胞生长 ,另取腹腔冲洗液作阳性对照。结果 应用 10 6个 /ml浓度的胃癌细胞液 ,气腹压达 30mmHg ,流量为 5L/min ,持续 5min、6 0min、12 0min及 180min后 ,部分培养瓶中发现有肿瘤细胞生长 ;而应用 10 4 个 /ml浓度的胃癌细胞液则未发现肿瘤细胞生长 (P<0 .0 0 1)。结论  10 6个 /ml胃癌细胞浓度下 ,高气腹压 ( 30mmHg)、时间 ≥6 0min可能造成肿瘤细胞的切口播散。本实验结果为临床上如何有效地预防腹腔镜手术切口的肿瘤细胞种植提供了参考依据 。 Objective To investigate the effect of CO 2 pneumoperitoneum on the tumor cell port site implantation in laparoscopic surgery. Methods Male Spraque Dawley rats were intraperitoneally injected with gastric cancer cells (cell line SGC 7901). Continuous CO 2 pneumo of 15 mm Hg or 30 mm Hg were established for 5 mins, 60 mins, 120 mins and 180 mins with the injection of different concentrations of tumor cells (10 4/ml, 10 6/ml respectively). Several samples of peritoneal washing served as positive control. All collecting dishes were incubated at 37℃ with 5% CO 2 concentration for one week and then examined for the presence of tumor cell under microscope. Results After one week of incubation, some of the dishes with continuous flow of CO 2 gas (5 L/min) at pneumo 30 mm Hg for 60 mins or longer demonstrated tumor growth, and all peritoneal washing samples showed tumor growth, while other dishes showed negative. Conclusion The research suggests that gastric cancer cells can cause port site implantation and the concentration of tumor cells, pneumoperitoneum pressure and duration may affect the occurrence of port site implantation. It may help to find a suitable way to prevent the port site implantation in operations.
出处 《中国普外基础与临床杂志》 CAS 2002年第1期16-18,共3页 Chinese Journal of Bases and Clinics In General Surgery
关键词 CO2气腹 胃癌细胞株 持续性气腹 切口种植 CO 2 pneumoperitoneum Gastric cancer cell Continuous pneumoperitoneum Port site implantation
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