新生儿网织红细胞参考值的动态变化及探讨

The reference limit of newborn's reticulocyte count and its dynamic variation

目的 探讨正常新生儿网织红细胞参考值范围的动态变化及与日龄的关系。方法 用Beckman -Coult公司的stks- 3B全自动血细胞分析仪对 115例 1～ 10d正常新生儿的静脉血网织红细胞值进行分析 ,并同时与国际血液标准化委员会 (ISCH)所推荐的网织红细胞活体染色显微镜计数 -Miller窥盘法进行比较 ,将所得结果进行统计学处理。结果 1d、2d、3d、4d与 5～ 7d新生儿组结果之间存在着高度显著性差异 (P <0 .0 1) ;5～ 7d网织红细胞值降至 0 .3%～ 1.1% ;8～ 10d的新生儿网织红细胞值与 5～ 7d新生儿网织红细胞值之间无显著性差异(P >0 .0 5 ) ;且血细胞分析仪测定法与Miller窥盘法测得新生儿网织红细胞值之间均无显著性差异 (P >0 .0 5 )。结论 新生儿网织红细胞参考值范围与日龄呈显著相关 ,并应注意其低值期的存在 ;

Objective To discuss the dynamic variation of the reference limit of newborn's reticulocyte count. Methods From 115 healthy newborns aged 1～10 d, the reticulocytes were counted with Beckman-Coult stks-3B automated blood cell counter, and the data were compared with that of Miller's method ( recommended by International Committee for Standardization in Haematology, ICSH). Results Obvious daily decrease was noticed in the first 5～7 neonatal days (P<0.01), with the reticulocyte count dropped from 4.081% to 0.714%. No further drop was found on the 8th～10th days, and there were no differences between the results obtained by automated blood cell counter method and Miller's method (P<0.05). Conclusion The results indicate that the reference limit of newborn's reticulocyte count is markedly related to the neonatal life in days. The presence of the low value phase is of clinical significance. The automated blood cell counter method can be adopted to substitute Miller's method.