人子宫内膜癌裸鼠模型的建立及其生物学特性的研究

Establishment and biological characteristics of nude mice xenograft tumor models of human endometrial carcinoma

目的 为子宫内膜癌的体内实验及临床研究提供动物实验模型。方法 将人子宫内膜癌手术切除标本移植于BALB/C(nu/nu)裸鼠皮下 ,传代移植成功后进行鼠间传代。结果 共进行了14例手术标本移植 ,原代移植成功率为 42 9% ,自第 5代以后 ,移植瘤的传代成功率均达到 10 0 0 %。至 2 0 0 1年 5月 ,共有 5株移植瘤连续传至 48～ 6 3代 ,连续传代的移植瘤仍保持原癌组织病理形态特点及人类肿瘤染色体的特点。在传代过程中 ,裸鼠模型SL 1、SL 2、SL 3的DNA倍体和DNA指数均无明显变化 ,而SL 4、SL 5随着传代次数的增加 ,DNA倍体和DNA指数均发生明显变化。 5个移植瘤模型雌激素受体 (ER)、孕激素受体 (PR)表达均为阴性 ;p5 3蛋白表达 :SL 3、SL 5阴性 ,SL 1弱阳性 ,SL 2、SL 4强阳性 ;P16表达 :SL 1、SL 4、SL 5阴性 ,SL 3弱阳性 ,SL 2强阳性 ;c erbB 2表达 :SL 3、SL 5阴性 ,SL 2弱阳性 ,SL 1、SL 4强阳性。结论 人子宫内膜癌手术标本经 48～ 6 3代传代 ,成功建立了 5株人子宫内膜癌裸鼠皮下移植瘤模型 ,为开展人类子宫内膜癌的体内实验及临床研究 。

Objective To establish nude mice xenograft tumor models of human endometrial carcinoma for basic and clinical study. Methods Fourteen samples of human endometrial carcinoma were subcutaneously heterotransplanted to nude mice (BALB/C, nu/nu),3～4 mice were transplanted for every sample. Results The initial take rate was 42.8%,then it increased to 100.0% after the 5th passage. Five of 14 samples were transplanted for 48～63 passages.The characteristics of histology,ultrastructure and chromosome were identical to those of human donour tumors. The DNA ploidy was consistent for SL-1,SL-2 and SL-3,but changed for SL-4 and SL-5 by cytometric analysis. The expression of estrogen receptor and progesterone receptor for 5 models was all negative. The expression of p53 was positive for SL-1,SL-2 and SL-4,but negative for SL-3 and SL-5.The expression of c-erbB-2 was positive for SL-1,SL-2 and SL-4,negative for SL-3 and SL-5.The expression of p16 was positive for SL-2 and SL-3,negative for SL-1,SL-4 and SL-5. Conclusions Five nude mice xenograft tumor models of human endometrial carcinoma were established successfully. It will be helpful for the basic and clinical study of human endometrial carcinoma.