海洋有毒甲藻Coolia monotis的研究 II.培养条件的测定

STUDY ON TOXIC MARINE DINOFLAGELLATE COOLIA MONOTIS MEUNIER Ⅱ. CONDITIONS FOR GROWTH

1 998年 5— 9月 ,通过培养实验 ,研究了海洋底栖性有毒甲藻Cooliamonotis人工培养生长所需的理化条件。结果表明 ,培养水体深度较小而水平横截面积较大有利于获得较高产量。温度 1 6— 3 2℃、盐度 2 4— 3 8、K培养液浓度系列 1 /8K— 2K、培养液pH为 6 .5— 9.5都可存活 ,最大指数期平均生长率为 0 .6 9division·d- 1 ,获得的最大细胞密度为 6× 1 0 4 cells·ml- 1 ,是一种适应性较强的微藻。保种条件以温度 2 4℃、盐度 3 4、培养液浓度K ,pH为8.0为佳。

Growth of a toxic benthic dinoflagellate was tested under a series of factors in batch culture. Growth of C. monotis can be affected by container shape, temperature, nutrient concentration, salinity and initial pH. Container with higher s/h ratio (s-interface area of the culture medium, h-height of the culture medium) was preferred. Temperature below 16℃ and up 30℃ limited division rate, the peak K occurred at 28℃. Optimum growth occurred at salinity 34, but it showed little differences among the six treatments. Cell density increased as the nutrient increased, density in 2K was the most, but K was suggested for practical small-scaled culture. Initial pH affected growth noticeably. The optimum pH was 8.0. The most biomass we estimated in the culture was 6×10 4cells·ml -1. Maximum average growth rate during potential phase was 0.69 division·d -1.