大鼠面神经再生过程中BMP-2、-6在面神经核内的定位表达及其作用

The expression of BMP-2 and BMP-6 in rat facial nerve motorneurons after facial nerve injury

目的 :探讨BMP 2 , 6在面神经损伤后再生过程中可能的作用。方法 :建立大鼠面神经切断吻合伤模型 ,利用免疫组化方法及图象分析系统 ,检测BMP 2、 6在面神经核团内的定位及表达变化。结果 :大鼠面神经运动神经元正常状态下可见BMP 2、 6表达。损伤发生后 :①BMP 2阳性表达呈下调型改变 ,6h时最低 ,与正常对照组相比有显著性差异 (P <0 .0 5 ) ;4周时恢复到接近正常表达水平 (P >0 .0 5 ) ;②BMP 6阳性表达呈现上调型改变 ,2周时达到最高值 ,4周时仍处于高表达状态 ,与正常对照组相比差异显著 (P <0 .0 5 )。结论 :BMP 2可能主要参与面神经损伤后早期应激及修复再生活动 ,BMP 6则可能参与晚期面神经再生。

?Objective: To study the effects of endogenous bone morphogenic protein 2(BMP 2) and 6(BMP 6) in rat facial nerve after injury. Methods: Facial nerve trunk was cut and then anastomosed in 30 adult male Sprague Dawley rats,another 6 rats without operation were used as the controls. Every 6 rats were killed 6 ,72 h,1, 2 and 4 weeks respectively after operation and nerve specimens were immunohistochemically examined for BMP 2 and BMP 6 expression. Result:In the control nerve the gray level of BMP 2 and BMP 6 were 210.89±8.21 and 232.03±9.25 respectively. 6, 72 h,1, 2 and 4 weeks after operation the gray level of BMP 2 were 242.83±11.01,240.67±7.91,234.46±5.28,232.12±7.27 and 220.71 ±10.19;that of BMP 6 210.75±5.19,204.08±10.85,198,91±8.58,186.37±4.11 and 184.62± 8.45 ,respectively. Conclusion: BMP 2 may play a role in the early irritable actions when facial nerve was injured. However, BMP 6 might have functions in the regeneration of facial nerve during later period after injury.