降钙素基因相关肽对人牙髓细胞的矿化影响

Effects of calcitonin gene-related peptide on calcification of human dental pulp cells in vitro

目的 :分析降钙素基因相关肽 (calcitoningene relatedpeptide ,CGRP)对人牙髓细胞促矿化功能的影响 ,探讨CGRP在牙髓炎症状态下表达量增多的生物学意义。方法 :人牙髓细胞分实验组和对照组培养 ,实验组加入适量的CGRP。分别测定细胞的碱性磷酸酶活性、骨钙素含量、I型胶原mRNA表达量变化。结果 :培养 3、6、9、12、15d ,5个时间点实验组比对照组细胞碱性磷酸酶活性增强、骨钙素含量增加 ,且有显著性差异 (P <0 .0 1)。培养 6d的实验组的细胞I型胶原mRNA表达比对照组明显高。结论 :CGRP刺激牙髓细胞分化 ,促进矿化形成 ,CGRP表达增高将有助于修复性牙本质形成。

砄bjective: To investigate the effect of calcitonin gene related peptide(CGRP) on calcification function of human dental pulp cells(HDPCs) in vitro . Methods: HDPCs cells were exposed to calcitonin gene related peptide at the dosages(mol/L) of 10 -12 , 10 -10 and 10 -8 for 3,6,9, 12 and 15 days respectively. Cell growth was studied by cell counting , alkaline phosphatase(AL) by a AL kit, osteocalin by immunoradiological tecnique and type I collagen mRNA expression by RT PCR. Results: CGRP did not show any effect on the growth of HDPCs, but it increased the expression of AL, osteocalin and type I collagen mRNA in HDPCs in a dose dependent and not time dependent manner. Conclusion: CGRP plays an important role in promoting differentiation and calcification function of HDPCs