RP-HPLC法测定不同药用部位不同采收期藤茶中蛇葡萄素的含量

Determination of Ampelopsin in the Different Parts of Ampelopsis grossedentata in Different Seasons by RP-HPLC

目的:运用RP-HPLC法测定不同采收期藤茶根、茎和叶中蛇葡萄素的含量。方法:使用ODS色谱柱(5μm,250 mm×4.6 mm),流动相为甲醇-水-磷酸(25:75:0.1),流速1.0ml/min,检测波长289 nm。结果:藤茶叶中蛇葡萄素含量最高,茎次之,根中最低;4月至5月间采集的藤茶中蛇葡萄素含量最高。

Objective:To determine ampelopsin in the root,stem and leaf of Ampelopsis grossedentata in different seasons by RP-HPLC. Methods:The determination was carried out with ODS column (5μm,250 mm×46 mm),using methanol-water-phosphoric acid(25:75:0. 1) as the mobile phase at a flow rate of 1.0 ml/min and detected at the wavelength 289 nm. Results:Ampelopsin content was in the order of leaf > stem> root. Ampelopsin content in Ampelopsis grossedentata collected in April and May was the highest.