丹参酮ⅡA对四氯化碳致大鼠肝纤维化的实验研究

Effect of Tanshinone ⅡA on CCl4-induced Liver Fibrosis in Rats

目的:探讨丹参酮ⅡA(Tanshinone ⅡA,Tsn)对四氯化碳(CCl_4)中毒性肝纤维化的治疗作用。方法:复制大鼠CCl_4肝纤维化模型,以马洛替酯为阳性对照,采用光镜观察组织学改变,测定血清谷丙氨酸转移酶(ALT)、谷草氨酸转移酶(AST)、一氧化氮(NO)、透明质酸(HA)、层粘连蛋白(LN),肝组织羟脯氨酸(Hyp)、丙二醛(MDA)以反映肝细胞损伤及肝纤维化的程度。结果:丹参酮ⅡA能降低实验性肝纤维化大鼠血清中升高的ALT、AST、NO、HA、LN水平和过高的肝组织中Hyp、MDA的含量。病理组织学检查亦表明,丹参酮ⅡA能明显改善实验性肝纤维化。结论:丹参酮ⅡA可能对实验性肝纤维化具有一定的治疗作用。

Objective:To study the effect of tanshinone ⅡA on liver fibrosis in rats induced by CCl4,. Methods:Model of liver fibrosis in rats were induced by subcutaneous injection of CCl4 and malotilate was as positive control. The histological changes were observed with light microscope. The levels of ALT,AST,NO,HA,LN in serum and Hyp,MDA in liver tissue were determined to reveal the event liver damage. Result:Tanshinone ⅡA could significantly reduce the elevate levels of ALT,AST,NO,HA,LN in serum and Hyp,MDA in liver tissue. Pathological examination suggested tanshinone HA has therapecuical effect on experimental liver fibrosis. Conclusion:Tanshinone ⅡA may have therapeutical effect on CCl4-induced liver fibrosis in rats.