淫羊藿总黄酮对体外培养成骨细胞的影响

Effects of Epimedium Pubescens Flavonoids on Osteoblasts in vitro

目的 :观察淫羊藿总黄酮对体外培养成骨细胞的作用。方法 :取新生 1～ 3d SD大鼠头盖骨用酶消化法分离成骨细胞。第 3代细胞经 MTT法、茜素红染色法观察淫羊藿总黄酮对体外培养成骨细胞增殖、矿化结节形成的影响。结果 :淫羊藿总黄酮 1～ 1 0 μg/ml浓度具有显著的促进成骨细胞增殖及提高矿化结节形成数量的作用。结论

AIM The purpose is to study the effects of Epimedium Pubescens Flavonoids on cultured osteoblasts in vitro . METHODS Osteoblasts were obtained from newborn rat calvaria by digestive enzymes. MTT, and ARS were used to observe the proliferation and the number of mineral nodes of the third passage osteoblasts cultured in vitro. RESULTS In 1～10 μg/ml concentration, Epimedium Pubescens Flavonoids significantly stimulated the proliferation and increased the number of mineral nodes of cultured osteoblasts. CONCLUSION Epimedium Pubescens Flavonoids had the effects on stimulating the proliferation and maturation of cultured osteoblasts in vitro .