山羊支原体的分离与鉴定

Isolation and Identification of Caprine Mycoplasmas

１９９７年对怀化市山羊疫病血清学调查时发现山羊传染性胸膜肺炎阳性率为７．２５％。为证实本病的存在，从１９９８年以来共采集山羊肺脏病理材料６０份，分离出二株支原体，将分离株接种山羊气管后复制出本病。根据国际兽疫局的标准诊断方法以及分离物的培养特性、显微镜观察、生长抑制试验、生化试验等，初步鉴定为丝状支原体山羊亚种和绵羊肺炎支原体。

The positive rate of caprine contagious pleuropneumonia was found to be 7.25% in a serological survey made in 1997 in Huaihua city of Hunan province by using indirect hemagglutination test. In confirmation of the existence of the disease, 60 caprine pathological lung tissues were collected mostly from this district since 1998. Two strains of mycoplasmas were isolated from the samples. According to OIE standard diagnosis procedures and the cultivated charaction, growth inhibition test, biochemical reaction of the isolated strains, the isolates were identified as M. mycoides subsp. capri . and M, ovipneumoniae.