摘要
研究了“美味樱桃番茄”的再生系统 ,以子叶盘和下胚轴切段为外植体 ,分析了不同激素及其质量浓度对外植体诱芽和诱根的影响 ,以及外植体对抗生素卡那霉素的敏感性。结果表明 ,适宜的诱芽培养基为 MS+ZT 2 .0 mg/L +IAA 0 .0 5 mg/L ,生根培养基均为 MS+IAA 0 .1m g/L ;卡那霉素筛选质量浓度为 5 0 mg/L。
The regeneration system of cherry tomato (cv.Delicious Cherry),cotyledons and hypocotyls used for explants,were examined.The result showed that the plant regulator played an important role in tissue culture.MS medium with ZT 2.0 mg/L+IAA 0.05 mg/L had great effects on the formation of shooting.The medium inducting root was MS+IAA0.1 mg/L,and the concentration of kanamycin for selecting transgenic tissue was 50 mg/L.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2002年第1期57-60,共4页
Journal of Northwest A&F University(Natural Science Edition)
基金
北京市科委重点实验室资助项目 ( 95 1890 2 11)
