光毒性试验方法研究

Study on Method for Phototoxicity Test

目的探讨采用单纯紫外线A(UVA)为照射光源的光毒性试验的影响因素及试验条件。方法观察了照射强度随照射时间的变化及照射时间对照射野内温度的影响。选择健康成年白化豚鼠 ,以10J/cm2 剂量UVA照射50min观察阳性对照8_甲氧基补骨脂 (8_MOP)的皮肤光毒性。结果照射开始后100min内光强度稳定在3 3～3.4mW/cm2 ,室温18℃时照射100min后照射野内温度上升1 5℃ ,8_MOP具有明显皮肤光毒性。结论该方法可准确测定化学物质的光毒性。

Objective To explore the influence factors and experimental conditions of phototoxicity test using pure UVA light source.Method The variations of light intensity associated with the exposure duration,the influence of exposure duration to UVA radiation on the temperature of the exposed area were both observed.The phototoxicity of positive control of 8_methoxypsoralen(8_MOP)to skin of healthy adult guinea pigs exposed to UVA radiation at dose of 10 J/cm2 for about 50min was observed also.Results The intensities of UVA light source were stable in the range of 3.3～3.4 mW/cm2 during the period of 100 minute_radiation.After a 100 minute_exposure to UVA radiation at 18 ℃,the temperature of the irridiated area increased 1.5 ℃,8_MOP revealed significant phototoxicity.Conclusion This method for phototoxicity test using pure UVA light source could examine the phototoxicity of a certain chemicals accurately,which was scientific and feasible.