5-HT_(1A)受体激动剂乌拉地尔对小鼠吗啡戒断反应与血浆及脑内NO含量的影响

Effect of Urapidil on Morphine Withdrawal Syndromes in Mice Changes of NO in Plasma and Brain Tissue

目的 :观察 5 -HT1A受体激动剂乌拉地尔 (urapidil)对小鼠吗啡戒断反应、血浆及脑内NO含量的影响。方法 :皮下注射定量吗啡 ,建立小鼠吗啡依赖模型。第 6天早 8:0 0用不同剂量的 5 -HT1A受体激动剂乌拉地尔给小鼠腹腔注射 (ip) ,8:2 0纳络酮催瘾 ,观察小鼠戒断时出现跳跃反应次数和体重丢失 ,评定小鼠戒断反应的强度。用硝酸还原法检测血浆和脑内NO的含量。结果 :三种不同剂量 (10 0 ,2 0 0 ,40 0mg kg-1)的乌拉地尔可抑制小鼠吗啡戒断反应 ,并在此剂量范围内呈量效关系。其中 40 0mg kg-1的乌拉地尔可使血中NO升高 ,但使脑内NO含量降低。结论 :5

Objective: To observe the effect of urapidil (agonist of 5-HT 1A receptor ) on morphine withdrawal syndrome Method: A physical dependent model in mice was established by subcutaneous injection of quantitative morphine The intensity of withdrawal syndrome was evaluated by observing jumping latency, jumping times and lose of body weight after intraperitoneal injection of naloxone (5 mg/kg) The effect of urapidil was observed after intraperitoneal injection it with 3 different dosages (100, 200, 400 mg/kg) Contents of NO in plasma and brain tissue were measured by nitrate reduction method Results: Morphine withdrawal syndrome was inhibited by urapidil at any dosage Content of NO in brain tissue reduced by urapidil 400 mg/kg, while plasma NO increased Conclusion: Urapidil inhibits morphine withdrawal syndrome in mice, increase the content of NO in their brain tissue