人骨髓基质干细胞体外培养的生物学特性

Biological characteristics of human bone marrow stromal stem cell cultured in vitro

目的 探讨在骨组织工程研究中作为种子细胞来源之一的骨髓基质干细胞体外培养后的生物学特性 .方法 获取人胎儿骨髓于含 10 0 m L· L- 1 胎牛血清的 DMEM培养液中培养 ;将骨髓基质干细胞向成骨细胞方向进行诱导 ,2～ 3wk后行钙化结节 Vonkossa和碱性磷酸酶 (AL P)钙钴染色 ;同时绘制诱导前后细胞的生长曲线、测定细胞内 AL P含量变化 ,并做统计学分析 .结果 骨髓基质干细胞经诱导后增殖速度变慢 ,但合成 AL P的能力明显增强 (P<0 .0 5 ) ;Vonkos-sa和 AL P染色呈阳性 .结论 骨髓基质干细胞取材方便 ,易于诱导为成骨细胞 ,作为骨组织工程的种子细胞应用前景良好 .

AIM To investigate the biological characteristics of human fetal marrow stromal stem cells in vitro . METHODS Human fetal bone marrow was obtained and cultivated immediately in DMEM medium containing 100 mL·L -1 fetal bovine serum. We induced the marrow stromal stem cells with mineralizational medium for 2~3 wk. Then the cells were stained by calcifying nodule Vonkossa and alkaline phosphatase (ALP) Calcium Cobalt methods. The cell growth curves were drawn. The content of ALP was mea sured and dealt with t test. RESULTS After inducing with mineralizational medium, the growth rate of the cells became slowly. The ability of secreting ALP was enhanced ( P < 0.05 ). And the results of stain reaction were positive. All these showed that marrow stromal stem cells had been differentiated determinately into osteoblasts. CONCLUSION Marrow stromal stem cell can be obtained and induced easily. The potential of its clinical application is forseeing.