人肝癌细胞系9724在不同免疫缺陷小鼠中的增殖特点

Model characteristics of human hepatocarcinoma cell strain 9724 proliferation in various strains of different immunodeficient mice

目的 探讨人类肝癌细胞 972 4在不同免疫缺陷小鼠的生长及模型特点 .方法 体外培养人类肝癌细胞 972 4;接种到不同免疫力小鼠 (B缺陷的 CBA/ N,T缺陷的 BAL B/ c裸鼠 ,T,B缺陷的 C.B- 17SICD小鼠及免疫重建的 SCID小鼠 .免疫正常的 BAL B/ c作为对照组 )皮下、肝内、腹腔 ,观察其生长特性 .结果 BAL B/ c小鼠和用 BAL B/ c外周淋巴细胞免疫重建的 SCID(BAL B/ c- PBL - SCID)不成瘤 ;CBA/ N小鼠随接种数量和途径不同而有不同的表现 ;BAL B/ c裸鼠 ,SCID小鼠和 CBA/ N小鼠外周淋巴细胞重建的 SCID(CBA/N- PBL- SCID) 10 0 %成瘤 .结论 SCID小鼠是建立肝癌模型的最佳小鼠 ,也是进行肿瘤免疫研究最佳模型动物 ;

AIM To explore the model characteristics of human hepatocarcinoma cells growing in various strains of different immunodeficient mice. METHODS The human hepatocarcinoma cells cultured in vitro were inoculated into va rious strains of different immunodeficient mice (B lymphocyte deficient mice-CBA/N, T lymphocyte deficient mice-BALB/c nu, T and B lymphocytes combined deficient mice-SCID and immunoreconstituted BALB/c PBL SCID, CBA/N PBL SCID. normal immune mice-BALB/c as controls) and their growths in the mice were surveyed. RESULTS There were no carcinoma growth in BALB/c and immunoreconstituted SCID mice with BALB/c mice peripheral blood lymphocytes (CBA/N PBL SCID); tumor growth in CBA/N mice was different with different cells inoculation sites; but there were 100% carcinoma growth in SICD, nude and immunoreconstituted SCID mice with CBA/N (CBA/N PBL SCID). CONCLUSION SCID mice are ideal animals for establishing hepatocarcinoma model and for studying tumor immunity. T lymphocytes play a main role in rejection to tumor.