cyclin D1、p53蛋白在胃癌组织中的表达

Expression of cyclin D1、p53 in Gastric Carcinoma

目的 研究cyclinD 1、p5 3蛋白在胃癌组织中的表达及两者的相关性。 方法 采用原位杂交技术及免疫组化技术 ,分别检测 73例胃癌组织中cyclinD1mRNA及 p5 3蛋白的表达。 结果 cyclinD 1在胃癌组织及癌旁组织中的阳性表达率分别为5 4.79% (4 0 /73 )及 2 8.77% (2 1/73 ) ,两者有显著性差异。cyclinD 1mRNA表达强度与肿瘤分化、浸润深度、淋巴结转移及病理分期无关 (P >0 .0 5 ) ,p5 3蛋白在胃癌组织中的阳性表达率为 3 2 .88% (2 4/73 ) ,cyclinD1表达强度与 p5 3表达无关 (P >0 .0 5 )。 结论 cyclinD1及 p5

Objective To investigate the expression of cyclin D1 gene and p53,and their relationship in gastric carcinoma.Methods In situ hybridyzation and immunohistochemical techniques were used to detect the expression of cyclin D1 mRNA and p53 protein.Results The positive rate of cyclin D1 mRNA in gastric carcinoma and normal tissue was 54.80% and 28.76% respectively,with significant difference( P <0.05).The cyclin D1 mRNA expression had no correlation to the tumor differenciation?tumor invasion?lymph node metastasis and pathologicl stage.The p53 positive rate was 32.87%.The expression of cyclin D1 mRNA was not associated with the p53 protein expression.Conclusion The overexpression of cyclin D1 and p53 play an important role seperately in the carcinogenesis of gastric carcinoma.