摘要
目的 探讨脾脏巨噬细胞 (M)在门静脉高压症 (PHT )脾功能亢进发生中的作用。 方法 采用免疫组化和透射电镜等方法 ,对比观察正常脾脏和PHT脾脏M的数量、分布、超微结构、M内酸性磷酸酶 (ACP)含量以及M吞噬破坏血细胞数量的变化。结果 与正常脾脏相比 ,PHT脾内M细胞密度相对减少 ,M内ACP含量增多 ,M表面伪足增多、增长 ,胞内溶酶体数量增多 ,M吞噬破坏血细胞的数量明显增多。 结论
Objective To investigate the role of splenic macrophage(M) in the occurrence of hypersplenism in patients with portal hypertension(PHT). Methods The changes in the quantity, distribution, ultrastructure of M, the quantity acid phophatase in M ,and the quantity of M phagocytizing blood cells were determined by immunohistochemistry and transmission electron microscopy in the normal and PHT spleen. Results Compared with normal spleen, the cell density of the M in PHT spleen decreased;and the quantity of acid phophatase in M increased; the surface folopodia of M increased and lengthened;and the quantity of lysosome in M and the quantity of M phagocytizing blood cells increased. Conclusions The splenic M may play an important role in the occurrence of hypersplenism in PHT.
出处
《中国普通外科杂志》
CAS
CSCD
2002年第1期20-22,共3页
China Journal of General Surgery