脾脏巨噬细胞在门静脉高压症脾功能亢进发生中的作用

Splenic macrophage in the occurrence of hypersplenism in patients with portal hypertension

目的 探讨脾脏巨噬细胞 (M)在门静脉高压症 (PHT )脾功能亢进发生中的作用。 方法 采用免疫组化和透射电镜等方法 ,对比观察正常脾脏和PHT脾脏M的数量、分布、超微结构、M内酸性磷酸酶 (ACP)含量以及M吞噬破坏血细胞数量的变化。结果 与正常脾脏相比 ,PHT脾内M细胞密度相对减少 ,M内ACP含量增多 ,M表面伪足增多、增长 ,胞内溶酶体数量增多 ,M吞噬破坏血细胞的数量明显增多。 结论

Objective To investigate the role of splenic macrophage(M) in the occurrence of hypersplenism in patients with portal hypertension(PHT). Methods The changes in the quantity, distribution, ultrastructure of M, the quantity acid phophatase in M ,and the quantity of M phagocytizing blood cells were determined by immunohistochemistry and transmission electron microscopy in the normal and PHT spleen. Results Compared with normal spleen, the cell density of the M in PHT spleen decreased;and the quantity of acid phophatase in M increased; the surface folopodia of M increased and lengthened;and the quantity of lysosome in M and the quantity of M phagocytizing blood cells increased. Conclusions The splenic M may play an important role in the occurrence of hypersplenism in PHT.