光动力疗法对大鼠类风湿性关节炎治疗作用的观察

Observation of the Effect of Photodynamic Therapy on Rat Rheumatoid Arthritis

目的 观察以血啉甲醚 (HMME)为光敏剂的光动力效应对类风湿性关节炎动物模型的治疗作用。方法 LEW大鼠 30只 ,随机分为正常对照组 5只、关节炎组 10只、治疗组 15只。关节炎组和治疗组用Ⅱ型胶原蛋白诱发关节炎 (CIA)。治疗组在大鼠出现踝关节红肿后 1周 ,炎症达到高峰时进行光动力治疗 (PDT)。随机治疗大鼠一侧的踝关节 ,对侧作为单纯HMME对照。治疗方法是大鼠麻醉后尾静脉注入HMME 10mg kg ,2 0min后踝关节照光 ,激光波长 6 2 7 8nm ,功率密度 10 0mW cm2 ,照射时间 10 0 0s,能量密度 10 0J cm2 。治疗后 2周取关节进行病理评分。结果 关节炎组滑膜细胞增生 ,血管翳形成 ,并侵蚀和破坏软骨和骨 ;治疗组滑膜细胞增生不明显 ,软骨和骨完整。病理评分治疗组滑膜增生、血管翳形成及软骨破坏、骨破坏和总分较关节炎组和HMME组低 ,统计学检验差异有显著意义 (P <0 0 1)。结论 在HMME 10mg kg,激光功率密度 10 0mW cm2 、能量密度 10 0J cm2 的条件下 ,光动力疗法能够有效地减轻CIA的滑膜细胞增生 ,减轻血管翳的形成及对软骨和骨的破坏 ,起到保护软骨和骨的作用。

Objective To investigate the effect of transdermal photodynamic therapy (PDT) on rheumatoid arthritis (RA) model in rats. Methods The collagen induced arthritis (CIA) rats were treated on day 7 after hind paw swelling and erythema. Animals were injected intravenously with HMME at a dose of 10 mg/kg. Twenty minutes later, one ankle of the rats randomly assigned was exposed to 267.8 nm laser irradiation at 100 mW/cm 2 for 1000 seconds, and another ankle was defined as of HMME group without laser. The other two groups are healthy control group and untreated CIA group. The histopathology of the ankle joint was assessed at day 21 after disease onset. Results The pathologic changes observed in the HMME group without laser and untreated CIA group included subsynovial inflammation, synovial hyperplasia, pannus formation, cartilage and bone destruction. The histological evaluation showed a statistically significant reduction in synovial hyperplasia, pannus formation and cartilage erosion, bone destruction and the total score in rats of PDT treated group. Conclusions The HMME mediated PDT may reduce synovial hyperplasia, pannus formation and cartilage erosion, and bone destruction in collagen induced arthritis in rats.