脑外伤患者血浆一氧化氮合酶与T淋巴细胞亚群的变化

Changes of plasma nitric oxide synthase and T lymphocyte sub-population in patients with craniocerebral injury

目的 :通过检测急性脑损伤患者血浆一氧化氮合酶 (NOS)与 T淋巴细胞亚群 (CD+4 /CD+8)含量变化 ,探讨其临床意义。方法 :采用酶法检测 NOS,应用单克隆抗体 APAAP法检测 45例脑损伤患者的 T淋巴细胞亚群。结果 :脑损伤患者血浆中 NOS与 CD+8细胞含量均较对照组升高 ,中、重型组差异有显著性 (P<0 .0 5或 P<0 .0 1) ;而脑损伤患者的 CD+4 较对照组下降 ,中、重型组差异有显著性 (P<0 .0 5或 P<0 .0 1)。 NOS与CD+8间呈正相关关系 (r=0 .36 7,P<0 .0 1)。CD+4 /CD+8比值与对照组比较差异均显著 (P均 <0 .0 1)。结论 :血浆中 NOS、CD+8升高水平与脑损伤程度有关 ,临床检测 NOS与

Objective:To investigate the clinical significance of nitric oxide synthase(NOS) and T lymphocyte subpopulation by measuring their concentration in blood.Methods:The concentration of NOS in plasma of 45 patients were measured with monoclone Alkaline phosphataseantakkaline phosphatase(APAAP).Results:Plasma NOS and CD + 8 lymphocyte subpopulation in patients with brain injury were increased respectively compare to the controls,especially in middle and severe patients( P <0 05 or P <0 01).While the CD + 4 subpopulation decreased respectively in middle and severe patievcts contrasted with control groups( P <0 05 or P <0 01).NOS had positive correlation with lymphocyte subpopulation CD + 8 ( r =0 367, P <0 01). CD + 4/CD + 8 were decreased respectively contrasted with control group(all P <0 01).Conclusions:The increased levels of NOS in plasma and CD + 8 have relation with the type and degree of brain injury.Measuring their concentration would contribute to assess the degree and the prognosis of the patients with brain injury.