制备抗原肽所用宫颈癌细胞培养方法的建立及HLA表达的检测

Establish a new method of cervical carcinoma cells culture for antigen peptide preparation and monitor the expression of HLA.

目的 探讨制备抗原肽所用宫颈癌细胞的培养方法及检测其HLA的表达。方法 先利用CO2孵箱和普通培养瓶进行培养 ,测定其生长状况 ,然后流式细胞仪检测HLA Ⅰ、Ⅱ类分子的表达 ,再用免疫荧光法检测不同浓度IFN γ对Hela细胞HLA Ⅰ、Ⅱ类分子的表达和IFN γ对不同培养时间Hela细胞HLA分子的表达。结果 IFN γ能够明显提高对数生长期的Hela细胞HLA Ⅰ类分子的表达 ,而IFN γ刺激前后Hela细胞HLA Ⅱ类分子的表达均为阴性。结论 本方法可缩短细胞培养时间 ,利用IFN γ诱导细胞提高HLA Ⅰ类分子的表达 ,为提供制备抗原肽所用宫颈癌细胞奠定了基础。

Objective To find a new culture procedure for Hela cervical carcinoma cells in vitro and check the HLA expression of the cells.Methods The cells were cultured in CO 2 incubator and status of growth was assayed. Then FCM was used in detecting the expression of HLA-Ⅰ and HLA-Ⅱ. Also the expression of HLA-Ⅰ was assayed after stimulated with INF-γ at different time point.Results We found the expression of HLA of Hela cells in log phase could be increased by INF-γ, whereas there was no HLA-Ⅱ expression before and after stimulated with INF-γ.Conclusion The methods used in our study shorten the culture time effectively. Meanwhile INF-γ can be applied to increase the expression of HLA-Ⅰ. All of this could provide a basis for preparation of antigen peptide from Hela cells.