Ⅱ型糖尿病血浆PAI-1、t-PA检测的意义和结果评价

Estimate the Result of Measuring PAI-1 and t-PA in NIDDM

目的:通过对Ⅱ型糖尿病患者分组(伴血管病变组、胰岛素抵抗组、胰岛素抵抗伴血管病变组、Ⅱ型糖尿病组)检测PAI-1、t-PA抗原及活性,并计算抗原比和活性比,以评价各检测指标的临床价值。方法:采用ELISA法检测PAI-1及t-PA抗原,采用发色底物法检测PAl-1、t-PA活性。结果:189例标本总体患病组及分组后各组的PAI-1活性、t-PA活性及t-PA活性/PAI-1活性比值与正常对照组比较均有显著性差异(P<0.05)。患病组间除t-PA抗原,其余检测显示胰岛素抵抗组、胰岛素抵抗组伴血管病变组与Ⅱ型糖尿病组存在显著性差异(P<0.05)。结论:在上述检测指标中t-PA活性、PAI-1活性反映血管病变的发生较敏感,t PA/PAI-1的活性比值更具临床应用价值。

Objective: To estimate the clinical application of antigen and activity of t-PA and PAI-1 . Methods: We studied 189 subjects of NIDDM and grouping(insulin resistance, coronary heart disease, insulin resistance with coronary heart disease, pure NIDDM), the concentrations and activities of t-PA and PAI-1 were measured using commercial kits. Results: There are differences between each NIDDM groups and normal group of activities of PAI-1 and t-PA, and also of the ratio of PAI-1 . Conclusion: The activities of PAI-1 and t-PA are more sensitivity in these index, and the ratios of t-PA/PAI-1 have more valve in clinical application