多发性尿路上皮肿瘤的单克隆起源

Monoclonal origin of multifocal urothelial carcinoma

目的 验证泌尿系上皮移行细胞癌单克隆起源学说。 方法 应用免疫组化染色方法 ,检测 10例 2 2块多发性尿路上皮移行细胞癌组织标本p5 3蛋白的表达 ,以此判断多发肿瘤灶基因的突变 ,比较多发肿瘤灶的基因型。 结果 同一患者不同部位肿瘤标本 p5 3染色阳性细胞的比例和染色强度完全一致 ,因此 ,肿瘤多克隆起源的可能性为 0 .5 1× 0 .5 1× 0 .5 2 × 0 .5 1× 0 .5 2 × 0 .5 1×0 .5 1× 0 .5 1× 0 .5 1× 0 .5 1=0 .5 12 =0 .0 0 0 12 2 (<10 -3 ) ,即同一患者多个肿瘤灶的基因型相同。

Objective To investigate the clonal origin of malignant cells in multifocal urothelial carcinoma. Methods p53 protein expressions were detected by immunohistochemistry in 22 pieces of tumor tissues from 10 patients with urothelial neoplasms.Mutation in p53 was inferred on the basis of immuno staining . Results The p53 protein expression of various tumors in the same patient completely matched, reflecting that the gene status of the multifocal tumor being the same. Conclusions The results strongly support the view that multifocal urothelial tumors are derived from the monoclonal cell.