脊髓损伤后胶质纤维酸性蛋白的表达及其意义

The Change of GFAP and Its Significance after Experimental Spinal Cord Injury

目的 :研究脊髓损伤 (SCI)后脊髓组织星形胶质细胞中胶质纤维酸性蛋白 (GFAP)表达的变化 ,探讨反应性胶质化在脊髓损伤中的作用。方法 :SD大鼠 2 5只 ,随机分成 5组 (n =5 ) :正常对照组、伤后 1天组、伤后 4天组、伤后 7天组和伤后 14天组 ,用免疫组织化学染色及图像分析的方法观察星形胶质细胞中GFAP的表达。用大鼠综合行为评分法 (CBS)对各级大鼠神经功能评分。结果 :SCI后星形胶质细胞中GFAP的表达与对照组比较明显增高 (P <0 .0 1) ,1～ 14天内呈进行性增高趋势 ;损伤各组的CBS 1～ 14天内呈降低趋势。两指标有显著的相关性 (r=- 0 .775 ,P <0 .0 0 1)。结论 :SCI后星形胶质细胞通过其反应性胶质化对脊髓的再生和自身修复起着重要作用。

Objective:To study the change of GFAP in spinal cord tissues after experimental spinal cord injury (SCI) and the role of reactive astrogliosis in injured spinal cord. Methods:Twentyfive SD rats were randomly divided into five groups: the groups of 1,4,7,14 day after spinal cord injury and control group ( n =5). Immunohistochemical technique and imagine analysis technique were used to detect the expressions of GFAP in spinal cord tissue, and the neurological function of spinal cord was graded according to Gale combined behavioral score (CBS). Results:The content of GFAP in astrocytes were much higher in groups of SCI than control group ( P <0.01), and the expression of GFAP showed increased tendency from 1st to 14th, however CBS showed decreased tendency. GFAP was significantly negatively correlated with CBS (r=-0.775, P <0.001). Conclusion:These data indicted that reactive astrogliosis may play an important role in self repairing and regeneration after SCI.