5/6肾大部分切除大鼠甲状旁腺细胞凋亡的变化及钙三醇的调节作用

Apoptosis of Parathyroid Cell in 5/6 Nephrectomized Rats and Its Change Induced by Calcitriol

目的：探讨慢性肾功能衰竭（ＣＲＦ）大鼠甲状旁腺细胞凋亡的变化以及钙三醇的调控作用。方法：研究对象为ＳＤ大鼠，分３组：Ａ组：ＣＲＦ组；Ｂ组：ＣＲＦ加用钙三醇组，每天皮下注射钙三醇５０ｎｇ；Ｃ组：假手术对照组。术后８周，采用流式细胞术检测大鼠甲状旁腺凋亡细胞比率（ＡＰＯ）和细胞周期，同时检测血清肌酐（ＳＣｒ）、Ｃａ＋｛２＋｝、Ｐ＋｛３－｝、碱性磷酸酶（ＡＬＰ）、全段甲状旁腺素（ｉＰＴＨ）以及尿内生肌酐清除率（ＣＣｒ）。结果：Ａ组和Ｂ组大鼠ＣＣｒ均较Ｃ组下降了近一半；Ａ组ＡＬＰ和ｉＰＴＨ显著高于Ｂ组和Ｃ组（％Ｐ％＜０．０１），而Ｂ组和Ｃ组差异无显著性（％Ｐ％＞０．０５）；Ａ组ＡＰＯ、Ｓ期细胞比率（ＳＰＲ）和增殖指数（ＰＩ）分别为（０．２３±０．１９）％、（１１．９９±２．７３）％和（１６．９３±３．４２）％，Ｂ组分别为（１．３２±０．５１）％、（２．９５±１．５８）％、（５．２４±２．２９）％，Ｃ组分别为（１．２９±０．４５）％、（０．７３±０．４６）％、（１．７６±０．７０）％，Ａ组ＡＰＯ显著低于Ｂ组和Ｃ组（％Ｐ％＜０．０１），Ａ组ＳＰＲ和ＰＩ显著高于Ｂ组和Ｃ组（％Ｐ％＜０．０１），但Ｂ组ＳＰＲ和ＰＩ仍高于Ｃ组?

Objective:To study the apoptosis of parathyroid cell in chronic renal failure (CRF) and observe its change induced by calcitriol. Methods:In the studies, Sprague-Dawley rats were divided into three grou ps (8 in each group). The five-sixth renal nephrectomy (NX) were performed in g roup A, 5/6 NX treated with calciriol 50 ng/d, subcutaneously administered for 8 weeks in group B and sham-operated control in group C. All parathyroid glands of rats were taken under anatomic microscope to evaluate. After 8 weeks apoptosi s rate (APO) and cell proliferative cycle of all parathyroid gland of rats were determined by flowmetry, meanwhile the serum Ca+{2+}, P+{3-}, ALP, iPTH an d urinary creatinine clearance (CCr) were also measured. Results:Both the 5/6 NX rats and 5/6 NX rats treated with calcitriol show remarkable decreasi ng in CCr compared to the sham-operated rats. Serum ALP and iPTH in group A wer e significantly higher than those of group B and group C. APO in group A was (0. 23±0.19)%, it was significantly lower than that of group B (1.32±0.51)% an d group C (1.29±0.45)%, respectively (P<0.01). While both S period cell rat e (SPR) a nd proliferation index (PI) in group A ［(11.99±2.73)% and (16.93±3.42)%］ we re sign ificantly higher than those in group B ［(2.95±1.58)% and (5.24±2.29%)］ a nd group Cu ［(0.73±0.46)% and (1.76±0.70)%］, P<0.01. But SPF and PI in g roup B were still hig her than in group C, P<{0.05}. Conclusion:The outstanding prolif eration of parathyroid cells may be partly given off by the reduction of its apoptosis a t least and calcitriol may induce apoptosis of parathyroid cells in 5/6 renal nephrectomy rats.